Zach Steinhart @CrisprEh
Postdoc in the @marsonlab @GladstoneInst/@UCSF. Parker Scholar @parkerici. Interested in genomics, CRISPR, cancer immunotherapy, and much more! 🇨🇦 San Francisco, CA Joined May 2021-
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Zach Steinhart retweeted
Excited to share that the Arce Lab is launching today at @arcinstitute ! We will explore the mechanisms controlling immune cell function using genomics and biochemistry in an effort to understand immune-mediated diseases. arcinstitute.org/labs/arcelab
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Zach Steinhart retweeted
Excited to share the latest from the @MarsonLab! An investigation of context-specific gene regulation in T cells, which led to dynamic mechanisms required to control T cell rest and activation. Published today in @Nature thanks to the work of many! nature.com/articles/s4158…
@EvanWeberPhD @Nature @alexdoan96 @kpmueller1 @andy_yhchen Congrats Evan! What a way to make a splash with your lab’s first paper!!
@DeAndyYang @Dev_journal @angerslab Congrats Andy!! Nice work!
@jcole_garcia @KoleRoybal @jaehyukchoimd @jay_daniels1 @Nature Congrats Julie!! Incredible work!
Zach Steinhart retweeted
Excited to share a milestone in our forward genetics developments! Today in @Nature we report massive mutagenesis screening in T cells with base editors, increasing resolution from genes to nucleotides nature.com/articles/s4158… A @MarsonLab story with my dear friend @carlcward 1/10
@CalebLareau @R_Schmidt_ @Nature @Satpathology Congrats Caleb! Great paper!
Zach Steinhart retweeted
I am very excited to share our paper on the interactions between #gdTcells and cancer cells, out today in @Nature! Using #CRISPR screens, we uncovered unexpected pathways in cancer cells regulating target molecules recognized by γδ T cells. @MarsonLab 1/2 nature.com/articles/s4158…
@CalebLareau @MSKCancerCenter Congrats!! Very well deserved! Also, nice website! I assume the “L”s represent mitochondrial genomes?
@oaaguilar503 @UCSF @immunox Congrats Oscar!!!
Zach Steinhart retweeted
Happy to share the newest paper from our lab. Led by technician extraordinaire Aidan Tousley (soon to be an MD/PHD student!), this manuscript details our attempts to reimagine the structure of CARs to improve their specificity and efficacy. rdcu.be/c7avS
Zach Steinhart retweeted
Thrilled to share the first Eyquem lab paper! Today we report in @CellCellPress evolving the AAV6 capsid to enable efficient engineering of murine T cells. An exciting collab with @asokanduke and led by the brilliants @WilliamNyberg and Jon Ark cell.com/cell/fulltext/… 🧵1/11
@kischober @JohnDoench @R_Schmidt_ @KrisHaurum @MangusoLab We oft observe the inverse, where some selected cells don't have kd w CRISPRi. Exosome/endosome transfer of guides is an interesting thought. We certainly see a lot of "soup" guides in our T cell perturb-seq suggesting intact guides are released from dying cells or secreted (2/2)
@kischober @JohnDoench @R_Schmidt_ @KrisHaurum @MangusoLab Interesting. My experience is mostly with puro selection on the sgRNA cassette, so I can't look at the negative population. My guess is that there is some low rate of recombination during lenti packaging where the GFP cassette is interrupted but the sgRNA cassette is intact (1/2)
@StephAngers @siobhanob29 @angerslab @UoftB @DonnellyCentre Congrats @siobhanob29 !!
Zach Steinhart retweeted
I’m hiring a computational biologist at Genentech to analyze cutting-edge single-cell genomics and high-throughput screening data in an extremely collaborative environment focused on finding cures for disease. Please reach out to learn more. careers.gene.com/us/en/job/GENE…
@KellyKersten8 @MaxKrummel Congrats Kelly!
Zach Steinhart retweeted
I'm very excited to share our review article rdcu.be/cHLHy @NatRevImmunol where we discussed how IL-2 has been engineered since its discovery to improve its in vivo activity and T cell selectivity and drive therapeutic efficacy in the context of autoimmunity and cancer.
@JohnDoench Such great libraries! And the PP7 SAM system was a huge boon too. So many great tools and discoveries coming out of your lab! 🙏
@chidiacrony @MarsonLab @ScienceMagazine @R_Schmidt_ Thank you Rony! Hope you are doing well!
Thrilled to have our recent work from the @marsonlab published in @ScienceMagazine science.org/doi/10.1126/sc…. This was the result of an awesome collaboration between myself and fellow Marson Lab postdoc, @R_Schmidt_ (1/12)
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